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Therapeutic effect of ANP on pulmonary microvascular endothelial cells injury induced by LPS
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.2005.04.001
- Key Word:
- 肺微血管内皮细胞;心钠素;脂多糖类;肺/损伤
Abstract： 目的: 探索心房钠尿肽(ANP)对脂多糖(LPS)引起肺微血管内皮细胞(PMVEC)损伤的治疗作用.方法: 培养大鼠PMVECs,分为对照组、LPS(1 mg/L)组和ANP治疗组(LPS+ANP),流式细胞技术检测各组细胞的周期,MTT比色试验检测细胞活力,并测试上清液中乳酸脱氢酶(LDH)活力和丙二醛(MDA)浓度.结果: ANP可以抑制LPS引起的S期和G2-M期细胞比例的降低.另外,LPS作用6 h后,0.01,0.1和1 μmol/L 3种浓度的ANP治疗和LPS作用0 、6 、12 和24 h后0.1 μmol/L 的ANP治疗,可以抑制LPS 引起的A490 nm值降低和LDH活力增强(P<0.05),治疗浓度越大,治疗越早,抑制作用越明显(P<0.05);MDA含量与LDH活力变化相似,仅24 h组无差异.结论: ANP可以减轻LPS引起的PMVECs损伤.
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