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Effects of interlukin-1 beta on retinas of rats with chronic glaucoma
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 2
- DOI:
- 10.3321/j.issn:1000-2790.2005.02.003
- Key Word:
- 白细胞介素-1;高眼压;视网膜;凋亡
Abstract： 目的: 研究慢性高眼压状态下大鼠视网膜中IL-1β表达水平的变化,探讨IL-1β在高眼压状态下对视网膜及节细胞的损伤作用.方法: 健康雌性SD大鼠40只,完全随机等分为正常组及慢性高眼压组,用巩膜浅层静脉烧烙法建立慢性高眼压模型.术后即刻、术后30 min,1 h,12 h,1 d,1 wk,4 wk,8 wk用TONO-PEN分别测量2组眼压.8 wk后处死大鼠,多聚甲醛灌流固定,恒冷箱切片机切片,厚度18 μm,贴于明胶玻片.用免疫组织化学的方法观察2组视网膜IL-1的表达及分布.结果: 高眼压组术后眼压值维持在4.04 kPa左右.IL-1β在正常组视网膜轻度表达,在高眼压组视网膜表达显著增强.结论: IL-1β参与了慢性高眼压状态下大鼠视网膜及视网膜节细胞损伤的病理过程.
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