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Comparative study of effect of PF4 and BMP-2m on hematopoietic damage in mice treated with γ-ray
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1000-2790.2005.01.001
- Key Word:
- 血小板因子4;骨形成蛋白-2;造血功能;辐射损伤
Abstract： 目的:比较PF4, BMP-2m及二者联用对大剂量射线照射后造血损伤的作用,比较二者的作用效果及有无协同作用. 方法:BALB/c小鼠36只,随机分成正常对照组、照射对照组、PF4, BMP-2m, PF4+BMP-2m联用1组和PF4+BMP-2m联用2组6组. 除正常对照组外,余30只用6.5 Gy 60Co-γ射线照射. 照射后10 d对动物的骨髓有核细胞数、脾结节、粒-巨噬细胞集落形成单位(CFU-GM)集落数和细胞周期进行检测. 结果:PF4组小鼠的骨髓有核细胞数显著高于BMP-2m组(P<0.05)、照射对照组和PF4+BMP-2m联用的两组(P<0.01). PF4组和BMP-2m组的脾结节数多于照射对照组(P<0.01). PF4+BMP-2m联用1组除了与PF4+BMP-2m联用2组的CFU-GM没有明显区别外,与其他各组相比均有非常显著的差别(P<0.01). PF4+BMP-2m联用2组的S期的DNA含量最高. 结论:各组对60Co-γ射线照射小鼠的造血损伤都具有显著保护和治疗作用. 但PF4和BMP-2m组的作用更好,未见明显协同治疗作用;联用2组各项数据除CFU-GM集落数外,明显比联用1组增高,如将联用组用药间隔的时间再调整,可能PF4+BMP-2m联用对造血细胞损伤的保护和促进修复增殖更有利.
- [1]刘斌,袁诚君,田琼,等.rhBMP-2m对化疗损伤小鼠的修复治疗作用[J].2003,(2).
- [2]田琼,张绍章,蒲勤,等.骨形成蛋白对小鼠造血型急性放射损伤治疗作用的研究[J].2001,(3).
- [3]韩忠朝,蔡英林,sAidoudi.血小板第4因子及四肽AcSDKP的造血保护作用研究[J].1999,(1).
- [4]杨岚,朱柏贵,田琼,等.血小板因子4的辐射防护作用[J].2003,(16).
- [5]Z C, Han,M, Lu,J, Li,etc.Platelet factor 4 and other CXC chemokines support the survival of normal hematopoietic cells and reduce the chemosensitivity of cells to cytotoxic agents.[J].1997,89(7).
- [6]S K, Gupta,J P, Singh.Inhibition of endothelial cell proliferation by platelet factor-4 involves a unique action on S phase progression.[J].1994,127.
- [7]F, Li,S, Lu,L, Vida,etc.Bone morphogenetic protein 4 induces efficient hematopoietic differentiation of rhesus monkey embryonic stem cells in vitro.[J].2001,98(2).
- [8]Kizaki M,Kawamura C,Ikeda Y.Bone morphogenetic protein (BMP)-2 induces apoptosis in human myeloma cells.[J].2002,43(3).
- [9]Z, Han,Y, Cai,S, Aidoudi.[The hemoprotective effect of platelet factor 4 (PF4) and tetrapeptide AcSDKP].[J].1999,20(1).
- [10]J, An,V, Rosen,K, Cox,etc.Recombinant human bone morphogenetic protein-2 induces a hematopoietic microenvironment in the rat that supports the growth of stem cells.[J].1996,24(7).