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In vitro culture and biological characteristics of human bone marrow-derived endothelial progenitor cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 20
- DOI:
- 10.3321/j.issn:1000-2790.2004.20.004
- Key Word:
- 骨髓;内皮/细胞学;干细胞;组织工程;种子细胞
Abstract： 目的:体外分离纯化人骨髓内皮前体干细胞,研究其基本生物学特性.方法:利用密度梯度离心法分离成人骨髓得到单个核细胞,再用内皮细胞条件培养基培养得到内皮前体干细胞,观察细胞生长特性,通过检测细胞血管内皮生长因子受体-2(VEGFR-2)、Ⅷ因子相关抗原抗体表达和透射电镜对培养细胞进行鉴定.结果:原代培养后细胞贴壁生长,7～10 d形成集落或融合呈卵石形,免疫组化荧光染色后结果显示VEGFR-2, Ⅷ/vWF 阳性,透射电镜显示细胞内具有特征性的W-P小体.结论:用密度梯度离心法和条件培养可以从骨髓得到高纯度内皮前体干细胞,该细胞具有与血管内皮细胞共同的特征,可以进一步分化为血管内皮细胞,是理想的组织工程种子细胞.
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