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Detection of persistent sodium current in injured DRG neurons
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 20
- DOI:
- 10.3321/j.issn:1000-2790.2004.20.001
- Key Word:
- 神经节,脊;膜片钳术;持续性钠流;阈下膜电位振荡
Abstract： 目的:检测损伤大鼠背根节(DRG)神经元持续性钠流(persistent sodium current, Inap)并研究其与阈下膜电位振荡的关系. 方法: 术后3～6 d慢性痛大鼠模型背根节急性分离,消化离散成单细胞,对中小型DRG神经元运用全细胞膜片钳技术记录. 结果: 电压钳下,钳制电位在-80 mV,给予斜波去极化到-30 mV时,细胞产生一持续时间150～200 ms,幅值89～200 pA的持续性内向钠流,且对100 nmol/L TTX敏感.电流钳下,产生阈下膜电位振荡的细胞,给予100 nmol/L TTX后,振荡消失.结论:在受损DRG神经元上检测到的持续性钠流可能是产生阈下膜电位振荡的基础之一.
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