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Induction of specific CTLs in BALB/c mice by chiral MUC1 synthetic peptide vaccine
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 16
- DOI:
- 10.3321/j.issn:1000-2790.2004.16.004
- Key Word:
- MUC1;手性;疫苗;T淋巴细胞,细胞毒性
Abstract： 目的: 研究由具有局部手性特征的合成多肽D-MUC1和小鼠血清白蛋白(mouse serum albumin,MSA)构成的多肽疫苗对小鼠MUC1特异性CTL的诱导作用. 方法: 用固相合成法合成含有D-Ser的多肽D-MUC1,纯化后与载体蛋白MSA偶联,分别以细胞因子重组人粒细胞-巨噬细胞集落刺激因子(GM-CSF)和胸腺肽(TP)为佐剂,免疫BALB/c小鼠,测定淋巴细胞增殖活性、MUC1特异性CTL杀伤活性. 结果: 合成肽经HPLC纯化后,质谱分析证明目的肽的分子量与理论值相符;D-MUC1多肽疫苗对BALB/c小鼠淋巴细胞增殖有明显的促进作用;D-MUC1多肽疫苗诱导出的特异性CTL杀伤MUC1阳性肿瘤细胞GZ.A5.3H.4. 结论: 研究结果表明D-MUC1合成肽疫苗可以在小鼠体内诱导出MUC1特异性CTL,为具有局部手性特征的MUC1合成肽疫苗进行肿瘤免疫治疗提供了实验依据.
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