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Migration and invasiveness of monocytes enhanced by human osteosarcoma cell line MG63 in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 14
- DOI:
- 10.3321/j.issn:1000-2790.2004.14.005
- Key Word:
- 骨肉瘤;单核细胞;侵袭
Abstract： 目的:观察骨肉瘤细胞对单核细胞侵袭能力的影响,探讨肿瘤浸润单核细胞协助肿瘤侵袭的作用.方法:利用transwell体外模型检测骨肉瘤细胞系MG63条件培养基作用下人单核细胞系Thp-1迁移及侵袭能力的变化;明胶酶谱方法检测MG63细胞条件培养基作用后Thp-1细胞表达MMP-9的改变.结果:与阴性对照组对比,MG63细胞条件培养基明显增强了Thp-1细胞迁移及侵袭能力;而且MG63细胞条件培养基对Thp-1细胞具有趋化作用.明胶酶谱结果提示MG63细胞条件培养基作用后Thp-1细胞表达MMP-9增强.结论:骨肉瘤细胞MG63能够增强单核细胞Thp-1的迁移及侵袭能力,并可能是通过增强单核细胞MMP-9的表达而实现的.
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