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Isolation, culture, and propagation of human umbilical cord blood PSC3445 pluripotent stem cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 11
- DOI:
- 10.3321/j.issn:1000-2790.2004.11.002
- Key Word:
- 脐血;多潜能干细胞;单个核细胞;细胞分化
Abstract： 目的: 培养、鉴定、描述及扩增人脐血PSC3445多潜能干细胞.方法: 使用Ficoll-Hypaque分离新鲜人脐血单个核细胞,用PBS洗涤2～3遍后在Potentculture完全培养液中37℃,50 mL/L CO2条件下培养10～14 d,得到一群成纤维样细胞,该细胞与荧光素标记的mAb (CD14-PE,CD11b-PE,CD45-FITC,CD34-PE,CD3-FITC,CD105-PE,CD166-PE,CD29-PE,CD90-FITC,CD33-PE,CD41-PE,CD44-FITC,CD7-FITC,CD61-FITC,HLA-DR-PE)反应后,经流式细胞仪检测其表面标志.并对该细胞进行了糖原(PAS),酸性磷酸酶(AP),碱性磷酸酶(Alk.P),油红O(ORO),过氧化物酶(POX)等化学染色.结果: PSC3445表达:HLA-DR+,CD14+,CD45+,CD11b+,CD29+,CD34-,CD105-,CD3-,CD90-,CD166-.细胞化学染色为PAS+,AP+,Alk.P-,ORO-,POX-.在适当诱导剂的诱导下可以定向分化.结论: 该细胞为存在于人脐血中一群尚未报道的多潜能干细胞.
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