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Co-immunoprecipitation for interactions of three human septin proteins
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1000-2790.2004.10.011
- Key Word:
- septin蛋白家族;免疫共沉淀;细胞周期蛋白质类
Abstract： 目的:探讨人肝癌中表达的3种septin 蛋白1, 2和6自身之间和相互之间是否存在相互作用关系,从而探讨在肝癌中septin家族的作用方式. 方法:通过构建septin家族基因的pCMV-Myc,pCMV-HA表达载体,采用人肾293细胞内蛋白的免疫共沉淀进行验证蛋白的相互作用. 结果:Septin 家族蛋白中确实存在着相互作用,不仅蛋白相互之间存在相互作用,且同一种蛋白质的分子之间也存在着较强的相互作用. 结论:Septin家族蛋白可能以复合物的形式在肿瘤发生过程中参与细胞活动.
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