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Role of potassium and chloride channel blockers in apoptosis of cardiomyocytes induced by staurosporine
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 9
- DOI:
- 10.3321/j.issn:1000-2790.2004.09.005
- Key Word:
- 钾离子通道阻断剂;氯离子通道阻断剂;心肌细胞凋亡;Caspase-3;staurosporine
Abstract： 目的:探讨钾、氯离子通道在心肌细胞凋亡过程中的调节作用与Caspase-3激活的关系. 方法:在staurosporine诱导原代培养鼠心肌细胞凋亡模型中,观察钾、氯离子通道阻断剂(Quinine, BaCl2, DIDS和NPPB)对心肌细胞的存活率、DNA片断、Caspase-3活性及细胞膜完整性的影响. 实验分为阴性对照组(无药物处理)、阳性对照组(staurosporine处理)与药物干预组(staurosporine加离子通道阻断剂). 结果:①钾、氯离子通道阻断剂能有效的抑制staurosporine诱导的心肌细胞凋亡. 与阳性对照组(29.8%)相比细胞的成活率在奎宁、氯化钡、DIDS和NPPB组分别是59.7%,47.2%,58.6%和60.7%,均有显著的增加(P<0.01). 相对应的DNA片断电泳显示:Quinine, BaCl2, DIDS和NPPB组均有显著的抑制DNA的降解. ②钾、氯离子通道阻断剂能有效的抑制Caspase-3活性的激活,与阳性对照组(600.4%)相比细胞的Caspase-3活性在Quinine, BaCl2, DIDS和NPPB组分别是184.2%,216.3%,175.6%和221.4%,均有显著的抑制(P<0.01). ③细胞膜完整性实验乳酸脱氢酶水平显示各组中细胞的坏死性死亡小于10%. 结论:在staurosporine诱导的心肌细胞凋亡模型中,钾、氯离子通道阻断剂能有效的抑制Caspase-3激活所介导的心肌细胞凋亡.
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