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Role of volume-regulated chloride channel in proliferation of human gastric carcinoma cell line SGC7901
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 6
- DOI:
- 10.3321/j.issn:1000-2790.2004.06.003
- Key Word:
- 胃肿瘤;细胞分裂;氯化物通道;膜片钳术
Abstract： 目的: 探讨容量调控氯通道在人胃癌细胞增殖中的作用. 方法: 应用细胞计数及MTT比色分析法观察细胞增殖情况;用流式细胞仪检测细胞周期时相;用膜片钳技术观察NPPB对容量调控氯通道的作用. 结果: NPPB (100 μmol/L)能够可逆性地抑制SGC7901细胞数及MTT吸光度值.经NPPB处理48 h 后,G1细胞比例比对照组明显增高.给予NPPB后可以抑制低渗激活的氯电流. 结论: 容量调控氯通道在人胃癌细胞系SGC7901的增殖中发挥重要作用.
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