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Inhibitory effects of antisense oligodeoxynucleotide bcl-2 on lens epithelial cells induced by galactose
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1000-2790.2004.05.004
- Key Word:
- 反义寡核苷酸;半乳糖;晶体上皮细胞
Abstract： 目的:探讨反义bcl-2寡核苷酸(bcl-2 ASODN)对半乳糖诱导的兔晶体上皮细胞(LEC)生长的影响. 方法:人工合成与bcl-2基因翻译起始区序列互补的寡核苷酸,用半乳糖和反义寡核苷酸处理培养的晶体上皮细胞,应用细胞计数法和MTT法观察反义bcl-2寡核苷酸对半乳糖诱导的晶体上皮细胞增殖的影响. 结果:2.5～10.0 μmol/L反义bcl-2寡核苷酸能抑制半乳糖诱导的晶体上皮细胞的增殖,10.0 μmol/L时作用较显著. 结论:反义bcl-2寡核苷酸对半乳糖诱导的白内障晶体上皮细胞的生长增殖有抑制作用.
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