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Promotive effect of ectogenic nitric oxide on differentiation of neural stem cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.2004.04.004
- Key Word:
- 一氧化氮;神经干细胞;硝普钠
Abstract： 目的: 探讨外源性一氧化氮(ectogenesis nitric oxide,NO)对培养状态下神经干细胞分化的影响. 方法: 用含EGF的条件培养基原代培养小鼠神经干细胞,在第5日时,实验组加入硝普钠(sodium nitroprusside SNP)作为NO的体外供体,对照组不加SNP. 24 h后都换成含血清的培养基. 比较两组细胞的分化情况. 结果: 在含血清培养基中培养24 h后,两组细胞均发生分化,但实验组经SNP作用后,细胞分化速度较对照组明显加快,突起较长. 结论: NO能促进神经干细胞的分化,并能促进细胞分化后轴突的发育.
- [1]林绿标,章翔,吴景文,等.小鼠神经干细胞中一氧化氮合酶的表达[J].2002,(23).
- [2]Cline H,Peunova N,Enikolopov G,etc.Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain.[J].2001,21(22).
- [3]G, Enikolopov,J, Banerji,B, Kuzin.Nitric oxide and Drosophila development.[J].1999,6(10).