You Position: Home > Paper
Ectogenic nitric oxide inhibits proliferation of neural stem cells in mice
( views:238, downloads:0 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.2004.04.003
- Key Word:
- 硝普钠;一氧化氮;神经干细胞;一氧化氮合酶
Abstract： 目的: 探讨外源性一氧化氮(NO)对小鼠神经干细胞(NSC)增殖的抑制作用. 方法: 用含表皮生长因子(EGF)的条件培养基原代培养NSC,在培养基中加入不同浓度的硝普钠(SNP)作为NO的体外供体. 24 h后用Griess还原法检测细胞上清液中NO的释放量;取一半细胞进行MTT试验,另一半细胞换成无SNP的条件培养基继续培养24 h,再行MTT试验. 另取经SNP抑制24 h后的NSC进行血清诱导分化试验和免疫组化试验检测其NOS表达. 结果: 24 h后细胞上清液中NO释放量随SNP浓度的增高而增加;MTT试验反映经SNP作用后的NSC活细胞数较对照组明显减少(P<0.01);经SNP作用后的NSC在解除抑制后仍能保持旺盛的增殖能力并能被诱导分化为神经细胞样细胞,这些NSC表达nNOS和eNOS,而不表达iNOS. 结论: 外源性NO对培养状态下的小鼠NSC有抑制作用.
- [1]杨立业,惠国桢,赵文娟,等.体外培养人类神经干细胞神经球中髓鞘的形成[J].2002,(1).
- [2]Truman JW,Champlin DT.Ecdysteroid coordinates optic lobe neurogenesis via a nitric oxide signaling pathway.[J].2000,127(16).
- [3]G, Enikolopov,J, Banerji,B, Kuzin.Nitric oxide and Drosophila development.[J].1999,6(10).
- [4]J N, Bates,M T, Baker,R, Guerra,etc.Nitric oxide generation from nitroprusside by vascular tissue. Evidence that reduction of the nitroprusside anion and cyanide loss are required.[J].1991,42 Suppl.
- [5]Cline H,Peunova N,Enikolopov G,etc.Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain.[J].2001,21(22).
- [6]Franke H,Bidmon H,Hilbig H,etc.Nitric oxide synthase isoenzymes during in vitro development of rat neuronal and human non-neuronal cells.[J].2001,297(1).
- [7]N, Peunova,G, Enikolopov.Nitric oxide triggers a switch to growth arrest during differentiation of neuronal cells.[J].1995,375(6526).