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Effect of ROS-generating system on nonenzymatic protein glycation
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 3
- DOI:
- 10.3321/j.issn:1000-2790.2004.03.005
- Key Word:
- 活性氧组分;非酶糖基化
Abstract： 目的: 研究不同的活性氧(ROS)激发体系对蛋白质非酶糖基化反应的影响. 方法: 以Horinchi法建立蛋白非酶糖基化反应体系,并将反应液分成对照组和不同的ROS激发组,分别向各组中加入不同浓度的H2O2,CHP和Vc/Fe2+等ROS激发剂,37℃孵育60 d,以荧光光谱扫描进行AGEs产物鉴定,并以激发波长(EX) 370 nm/发射波长(EM) 440 nm测定各组荧光值. 结果: 荧光扫描显示,各组主峰均在EM 440～450 nm波谱范围内. 各ROS激发组荧光值明显低于对照组(P<0.05). 不同H2O2浓度下测定的荧光值两两比较显示,除20 μmol/L和80 μmol/L浓度下无显著性差别(P>0.05)外,各浓度组之间均有显著性差别(P＜0.05);过氧基异丙苯(CHP)和Vc/Fe2+ ROS激发体系所测定的荧光值之间也存在显著差别(P＜0.05). 结论: 在本研究的给定条件下,H2O2及CHP和Vc/Fe2+ ROS激发体系对体外蛋白质非酶糖基化反应具有明显的抑制作用;H2O2对蛋白非酶糖基化的影响具有一定的剂量效应关系;不同种类的ROS激发体系对蛋白质非酶糖基化反应有明显差异.
- [1]杨瑞华,李瑞珍,海春旭.VitC/Fe2+对细胞生长状态的影响[J].1999,(9).
- [2]吴凤兰,张亚非,张明珠.硒和维生素E对糖尿病大鼠氧化和非酶糖化的影响[J].1999,(2).
- [3]S, Horiuchi,N, Araki,Y, Morino.Immunochemical approach to characterize advanced glycation end products of the Maillard reaction. Evidence for the presence of a common structure.[J].1991,266(12).
- [4]G, Münch,R, Keis,A, Wessels,etc.Determination of advanced glycation end products in serum by fluorescence spectroscopy and competitive ELISA.[J].1997,35(9).
- [5]S, Asgary,G, Naderi,N, Sarrafzadegan,etc.Anti-oxidant effect of flavonoids on hemoglobin glycosylation.[J].1999,73(5).
- [6]Brownlee M.Advanced protein glycosylation in diabetes and aging.[J].1995,46(0).
- [7]M, Brownlee,A, Cerami,H, Vlassara.Advanced glycosylation end products in tissue and the biochemical basis of diabetic complications.[J].1988,318(20).
- [8]Balamurugan R,Nalini P,Sen SK,etc.Increased protein glycation in non-diabetic pediatric nephrotic syndrome: Possible role of lipid peroxidation.[J].2003,337(1).
- [9]L M, Vervoort,J E, Ronden,H H, Thijssen.The potent antioxidant activity of the vitamin K cycle in microsomal lipid peroxidation.[J].1997,54(8).
- [10]A, Bierhaus,M A, Hofmann,R, Ziegler,etc.AGEs and their interaction with AGE-receptors in vascular disease and diabetes mellitus. I. The AGE concept.[J].1998,37(3).
- [11]Peynet J,Walrand S,Raji B,etc.An intracellular modulation of free radical production could contribute to the beneficial effects of metformin towards oxidative stress.[J].2003,52(5).
- [12]T G, Lukovits,T M, Mazzone,T M, Gorelick.Diabetes mellitus and cerebrovascular disease.[J].1999,18(1).
- [13]Nobuaki Tabata,Yoshiaki Ito,Naoyuki Nishizawa,etc.Inhibition of glycation reaction in tissue protein incubations by water soluble rutin derivative[J].2003,249(1).
- [14]张文清,海春旭,龚书明.锌对大鼠肝微粒体脂质过氧化模型抗氧化作用的实验研究[J].1998,(4).
- [15]杨瑞华,李瑞珍,海春旭.过氧基异丙苯对细胞生长状态的影响[J].1999,(2).