You Position: Home > Paper
Apoptosis of mucoepidermoid carcinoma cells induced by recombinant human tumor necrosis factor-α
( views:170, downloads:4 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1000-2790.2004.01.005
- Key Word:
- 重组人肿瘤坏死因子-α;凋亡;黏液表皮样癌细胞
Abstract： 目的: 探讨重组人肿瘤坏死因子-α(rhTNF-α)作用于人黏液表皮样癌细胞(MEC-1)的效应与机制. 方法: 应用细胞记数、DNA降解片段琼脂糖凝胶电泳观察、电镜观察和细胞周期测定rhTNF-α作用后MEC-1细胞的凋亡状况. 结果: rhTNF-α作用于MEC-1细胞后,导致细胞存活数目减少,大量细胞发生凋亡;基因组DNA分解成寡聚核苷酸片段,DNA琼脂糖凝胶电泳有梯形条带;细胞周期发生改变,形态学观察细胞核皱缩,染色质边集. 结论: rhTNF-α是通过MEC-1细胞发生凋亡而发挥细胞毒作用.
- [1]欧新荣,沈子华,贺致敏.肿瘤坏死因子、干扰素协同化疗药物抑制舌癌细胞生长的研究[J].2000,(4).
- [2]李焰,吴军正,司徒镇强,等.人涎腺粘液表皮样癌耐药细胞系的建立及其生物学特性[J].2002,(18).
- [3]Schlag PM,Schumann R,Kettelhack C,etc.Pentoxifyllin Attenuates the Systemic Inflammatory Response Induced During Isolated Limb Perfusion With Recombinant Human Tumor Necrosis Factor-alpha and Melphalan.[J].2003,10(5).
- [4]Fiers W,Vanden-Berghe T,Vandenabeele P,etc.Structure/Function analysis of p55 tumor necrosis factor receptor and fas-associated death domain. Effect on necrosis in L929sA cells.[J].2000,275(48).
- [5]司徒镇强.人粘液表皮样癌细胞系MEC-1的建立及其生物学特性[J].1990,(2).
- [6]Hailing Hsu,Hong-Bing Shu,Ming-Gui Pan,etc.TRADD-TRAF2 and TRADD-FADD Interactions Define Two Distinct TNF Receptor 1 Signal Transduction Pathways[J].1996,84(2).
- [7]G H, Wong,R L, Kaspar,G, Zweiger,etc.Strategies for manipulating apoptosis for cancer therapy with tumor necrosis factor and lymphotoxin.[J].1996,60(1).
- [8]Christoph Borner,Isabelle Martinou,Chantal Mattmann,etc.The Protein bcl-2α Does Not Require Membrane Attachment, but Two Conserved Domains to Suppress Apoptosis[J].1994,126(4).
- [9]Terlikowski S,Sulkowska M,Nowak HF.The effect of recombinant human tumor necrosis factor-alpha on Ehrlich ascites tumor growth.[J].2002,21(1).
- [10]PA Noojn,R Manusama,AMM rmon,etc.Synergistic effects of TNF-α and melphalan in an isolated limb perfusion model of rat sarcoma: a histopathological, immunohistochemical and electron microscopical study[J].1996,74(12).
- [11]Ernstoff MS,Nadler PI,Wood DL,etc.Biologic response modulation by tumor necrosis factor alpha (TNF alpha) in a phase Ib trial in cancer patients.[J].1997,20(5).