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Cloning and sequencing of human heparanase gene from hepatoma

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Author:
No author available
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
1
DOI:
10.3321/j.issn:1000-2790.2004.01.004
Key Word:
乙酰肝素酶;硫酸乙酰肝素;基因工程;肝癌细胞

Abstract: 目的: 研究乙酰肝素酶在人肝癌细胞生长和转移中的作用,并自人肝癌组织中克隆该酶cDNA全长编码片段. 方法: 提取人肝癌细胞总RNA,以RT-PCR方法获取了乙酰肝素酶cDNA片段,将其与pGEM-T Easy载体连接,进行克隆及酶切鉴定. 结果: 序列分析表明,与GenBank中的人乙酰肝素酶全长cDNA序列比较,仅16个碱基不同,同源性为0.991. 结论: 自人肝癌组织中成功克隆了乙酰肝素酶cDNA全长编码片段.

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