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Effects of basic fibroblast growth factor on proliferation and differentiation of human periodontal ligament cells in vitro

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Author:
No author available
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
23
DOI:
10.3321/j.issn:1000-2790.2003.23.005
Key Word:
碱性成纤维细胞生长因子;牙周膜细胞;凝集素受体;体外

Abstract: 目的: 观察人重组碱性成纤维细胞生长因子(human basic fibroblast growth factor, hbFGF)对人牙周膜细胞(periodontal ligament cells, PDLC)增殖和分化的效应. 方法:对hbFGF (50 μg*L-1)刺激后3, 9, 15和21 d的细胞进行计数,同时行HE和免疫组织化学染色. 结果:hbFGF (50 μg*L-1)可促进PDLC细胞生长,在培养的3, 9, 15和21 d时,其细胞计数分别是38×103, 80×103, 106×103, 99×103. 但不改变PDLC的形态和生长方式;与对照组相比PDLC的ConA, WGA凝集素受体表达无差异. 两组细胞均未出现钙化现象. 结论:hbFGF可促进体外PDLC的增殖,本实验未发现hbFGF对细胞的促分化效应.

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