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Change of membrane dynamic characteristics and oxygen carrying ability in erythrocytes with different pH
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 23
- DOI:
- 10.3321/j.issn:1000-2790.2003.23.003
- Key Word:
- 快速显微多道分光光度;显微图像分析;红细胞;pH值;膜动态特性;携氧能力
Abstract： 目的: 研究单个活态人血红细胞在不同pH值(pH 5.8 ～ 9.0)下的膜动力学特性与携氧能力. 方法: 用新型快速显微多道分光光度技术和显微生物医学图像分析技术(fast multi-channel micro-spectrophotometer and image analysis techniques, FMIA)对红细胞形态结构、弯曲弹性模量(KC)、剪切弹性模量(μ)和吸光度(A)进行无扰、在位、实时、高灵敏度联合动态连续测量. 结果: 细胞形态随pH增大而减小;KC, μ和形状因子(roundness factor, REF)在pH 7.4～7.6时最小,随酸碱度增大而增大;A在pH 7.4最大,随酸碱度增大而减小. 结论: pH值与细胞的形态、膜的变形能力、流动特性和胞内血红蛋白分子氧合能力密切相关.
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