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Regulatory effects of electroacupuncture at Tsusanli on TNF-α in rats with ulcerative colitis
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 23
- DOI:
- 10.3321/j.issn:1000-2790.2003.23.001
- Key Word:
- 电针;足三里;结肠炎,溃疡性;肿瘤坏死因子-α
Abstract： 目的: 探讨电针足三里穴对溃疡性结肠炎(ulcerative colitis, UC)大鼠肿瘤坏死因子-α(TNF-α)的调节作用及机制. 方法: 将32只SD大鼠随机分为4组: 正常对照组,UC模型对照组,UC模型+针穴组,UC模型+非穴组. 在诱导产生UC大鼠模型的基础上,分别电针其相应部位,10 d后同时处死4组大鼠,观察其结肠质量、结肠组织髓过氧化物酶(MPO)活性及TNF-α mRNA表达水平、血清TNF-α含量的变化. 结果: 与正常对照组相比,UC模型对照组结肠质量/体质量(mC/mB)及MPO活性均显著增加(8.53±2.59 vs 2.46±0.42, 145.2±25.3 vs 24.3±7.8, P<0.01); 血清TNF-α含量和结肠组织TNF-α mRNA表达水平分别上升2.5倍及4.3倍(2278±170 vs 894±248, 0.98±0.11 vs 0.23±0.11, P<0.01); 电针足三里穴后, mC/mB和MPO活性显著降低(5.29±2.04 vs 8.53±2.59, 103.5±35.7 vs 145.2±25.3, P<0.01, 0.05); TNF-α含量和TNF-α mRNA表达水平也分别减少16%和44%(1913±232 vs 2278±170, 0.55±0.13 vs 0.98±0.11, P<0.01). 非穴组与UC模型对照组相比无统计学显著性差异(P>0.05). 结论: 电针足三里穴能够良性下调TNF-α生成及表达,可能是其治疗UC作用机制之一.
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