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Effects of exogenous p21WAF1/CIP1 gene on the growth and cell cycle of human glioma cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 22
- DOI:
- 10.3321/j.issn:1000-2790.2003.22.005
- Key Word:
- p21WAF1/CIP1基因;神经胶质瘤;细胞周期;脱噬作用
Abstract： 目的:探讨外源性p21WAF1/CIP1基因对胶质瘤细胞生长和细胞周期的影响.方法:采用脂质体介导法将p21WAF1/CIP1基因质粒转导入人胶质瘤细胞系SHG44细胞,然后用电镜、流式细胞仪等技术对细胞形态、生长及细胞周期进行观察.结果:酶切电泳和斑点杂交显示p21WAF1/CIP1基因成功的转染至SHG44细胞.转染p21WAF1/CIP1基因的细胞光镜及电镜下可见凋亡产生;生长速度明显慢于亲代细胞,其倍增时间约是对照细胞的二倍;细胞周期发生明显改变,G1期明显增多,S期明显减少.结论:外源性p21WAF1/CIP1基因对SHG44细胞的生长和细胞周期有明显的抑制作用,并可诱导细胞凋亡产生.
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