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Primary study on cyclin D1 expression pattern in human glioma cell line by double parameter flow cytometry
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 22
- DOI:
- 10.3321/j.issn:1000-2790.2003.22.003
- Key Word:
- 神经胶质瘤;细胞周期蛋白D1;细胞周期
Abstract： 目的:探讨cyclin D1在胶质瘤细胞系中的细胞周期表达模式,将为从生物学功能上阐明cyclin D1在胶质瘤细胞周期演进中的作用.方法:利用流式细胞仪双参数法和细胞同步化,确定cyclin D1在胶质瘤细胞系SHG-44和BT-325中的细胞周期表达分布模式.结果:在胶质瘤细胞系SHG-44和BT-325中,CyclinD1的表达呈细胞周期依赖性:在SHG-44中,cyclin D1在G1期表达最高,4 h左右达到峰值,S期及G2/M期下降,但仍可检测到;在BT-325中,cyclin D1在G1期表达最高,6 h左右达到峰值,S期及G2/M期下降,但仍可检测到.结论:cyclin D1蛋白质的表达呈细胞周期依赖性,且与细胞周期行进有关;cyclin D1在SHG-44和BT-325胶质瘤细胞进入G1期的早期发挥着重要的生物学作用.
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