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Establishment of a rat C6 brain-glioma model and observation of its cyclin D1 expression
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 22
- DOI:
- 10.3321/j.issn:1000-2790.2003.22.002
- Key Word:
- 神经胶质瘤;立体定位技术;细胞周期蛋白D1;大鼠
Abstract： 目的:建立类似人脑胶质瘤动物模型,观察cyclin D1在肿瘤组织中的表达.方法:借助动物立体定向仪,将体外培养大鼠C6胶质瘤细胞接种于Wistar大鼠左侧尾状核区.接种后观察实验大鼠生存状态,分别于接种后7,15 d进行MRI检查.对每例荷瘤鼠死后进行解剖,行组织病理学和GFAP免疫组化检查.随后对不同存活期大鼠cyclin D1表达进行检测.结果:该方法建立的胶质瘤动物模型在组织病理学上接近人脑胶质瘤.而且具有颅内生长稳定,成瘤率高,重复性好.在每例胶质瘤组织中cyclin D1均呈阳性表达,主要在细胞胞核表达,不同存活期荷瘤鼠其瘤组织中cyclin D1表达量不同,存活时间较长者,其表达量明显减少.结论:此类模型肿瘤生长特性及病理特征与人脑胶质瘤相似.cyclin D1表达量与脑胶质瘤大鼠存活时间呈负相关.
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