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Effect of PKC signaling pathway on VEGF expression in human retinal pigment epithelium cells under hypoxia
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 21
- DOI:
- 10.3321/j.issn:1000-2790.2003.21.005
- Key Word:
- 视网膜色素上皮细胞;血管内皮生长因子;蛋白激酶C;缺氧
Abstract： 目的: 探讨蛋白激酶C(PKC)信号传导通路对缺氧状态下培养的视网膜色素上皮(retinal pigment epithelium,RPE)细胞表达VEGF的作用. 方法: 在缺氧状态下分别培养人RPE细胞6, 12和24 h;将不同浓度的PKC激动剂佛波醇-12-豆蔻酰-13乙酸 (phorbol 12-myristate 13-acetate,PMA)及PKC抑制剂Chelerythrine分别加入人RPE细胞培养液,在缺氧状态下培养24 h. 用免疫组化方法检测各组RPE细胞中VEGF的表达, 经计算机图像处理, 定量分析. 结果: 在缺氧状态下,RPE细胞对VEGF的表达较非缺氧组RPE细胞显著增加(P<0.01);较对照组,PMA可增强缺氧状态下RPE细胞VEGF的表达(P<0.01), Chelerythrine则减弱VEGF的表达(P<0.01). 结论: 缺氧诱导VEGF在RPE细胞中的表达, 其信号传导途径部分是通过PKC通路实现的.
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