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Expression of adenovirally delivered BDNF in murine retina
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 21
- DOI:
- 10.3321/j.issn:1000-2790.2003.21.004
- Key Word:
- 视神经;脑源性神经营养因子;转染
Abstract： 目的: 研究腺病毒介导脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)基因转染在活体大鼠视网膜的表达. 方法: 将BDNF腺病毒注入大鼠玻璃体内,于不同时间点免疫荧光染色检测表达;酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测转染因素、损伤因素对视网膜表达BDNF的影响. 结果: 免疫荧光染色显示3 d节细胞即出现绿色荧光,可持续4 wk,对照组荧光着色细胞数、荧光强度均低于各时间点转染组;ELISA显示经BDNF转染各组在各时间点表达均显著高于非转染组(P<0.01),损伤非转染组在3 d和1 wk时高于正常组(P<0.01). 结论: 腺病毒介导BDNF基因转染可在大鼠视网膜有效表达,损伤可以导致早期的BDNF表达升高.
- [1]陈谦,汪家政,刘红,等.人脑源性神经营养因子及神经营养素3重组腺病毒的构建及表达[J].2000,(2).
- [2]S, Chierzi,J W, Fawcett.Regeneration in the mammalian optic nerve.[J].2001,19(1-2).
- [3]Isenmann S,Engel S,Gravel C,etc.Intravitreal adenoviral gene transfer evokes an immune response in the retina that is directed against the heterologous lacZ transgene product but does not limit transgene expression.[J].2001,892(2).
- [4]Bhattacharya SS,Reichel MB,Baker D,etc.An immune response after intraocular administration of an adenoviral vector containing a beta galactosidase reporter gene slows retinal degeneration in the rd mouse.[J].2001,85(3).