You Position: Home > Paper
B-cell maturation antigen can induce NF-κB activation
( views:202, downloads:37 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 19
- DOI:
- 10.3321/j.issn:1000-2790.2003.19.001
- Key Word:
- B细胞成熟抗原;NF-κB;转录因子
Abstract： 目的: 研究细胞表面B细胞成熟抗原(B-cell maturation antigen, BCMA)在B细胞激活因子刺激下,对转录因子NF-κB是否具有激活作用. 方法: 采用电转移基因转染和卤化钙基因转染方法,将BCMA和C末端缺失80个氨基酸残基的BCMA基因(BCMAΔC80)分别转染3T8细胞及kb gfp细胞,观察与NF-κB启动子下游的报告基因表达水平的变化. 结果: BCMA在3T8细胞中经BAFF激活后可上调报告质粒中CD14基因的表达,而C末端缺失80个氨基酸残基的突变BCMA(BCMAΔC80)则不具备此功能. 同时在另一报告细胞系kb gfp中亦观察到类似作用. 结论: 细胞表面的BCMA在被B细胞激活因子刺激后,可引起NF-κB的活化,这一作用依赖于BCMA的C末端的80个氨基酸残基.
- [1]J A, Gross,J, Johnston,S, Mudri,etc.TACI and BCMA are receptors for a TNF homologue implicated in B-cell autoimmune disease.[J].2000,404(6781).
- [2]Y, Laabi,M P, Gras,J C, Brouet,etc.The BCMA gene, preferentially expressed during B lymphoid maturation, is bidirectionally transcribed.[J].1994,22(7).
- [3]Hatzoglou A,Tsapis A,Bourgeade MF,etc.TNF receptor family member BCMA (B cell maturation) associates with TNF receptor-associated factor (TRAF) 1, TRAF2, and TRAF3 and activates NF-kappa B, elk-1, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase.[J].2000,165(3).
- [4]Chen Kiang S,Do RK.Mechanism of BLyS action in B cell immunity.[J].2002,13(1).
- [5]Xu S,Lam KP.B-cell maturation protein, which binds the tumor necrosis factor family members BAFF and APRIL, is dispensable for humoral immune responses.[J].2001,21(12).