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Isolation, cultivation and differentiation of stem cells from mouse dental pulp
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- 10.3321/j.issn:1000-2790.2003.18.004
- Key Word:
- 干细胞;牙髓干细胞;细胞培养;分化;牙本质涎磷蛋白
Abstract： 目的: 分离、培养小鼠牙髓干细胞并诱导分化. 方法: 采用酶消化培养法获得小鼠的单个牙髓细胞悬液,细胞密度调整为1×104 /孔细胞,用200 mL*L-1胎牛血清的α-MEM培养液培养14 d,挑出细胞克隆消化传代,TGF-β、BMP2、bFGF细胞因子诱导,PCR检测DSPP的mRNA的表达. 结果: 小鼠牙髓细胞呈集落状生长, 克隆形成率为 1.6-2.5个/104细胞,所形成的集落细胞结合紧密,细胞体积小、胞核大,经细胞因子刺激后的细胞表达DSPP的mRNA. 结论: 培养的小鼠牙髓集落状生长的细胞具有干细胞增殖快等特性,细胞因子可诱导小鼠牙髓干细胞定向分化.
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