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Difference of epidermal stem cells in adult and fetus skin
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- 10.3321/j.issn:1000-2790.2003.18.003
- Key Word:
- 表皮干细胞;表皮;角蛋白;整合素
Abstract： 目的: 检测表皮干细胞相对特异性分子角蛋白19(cytokeratin 19 Ck19)及整合素β1在胎儿及成人不同部位皮肤中的表达,以比较表皮干细胞在成人及胎儿皮肤中的差异.方法: 免疫组化方法检测Ck19及整合素β1,以及角朊细胞分化的特异标志分子角蛋白10(Ck10)在成人及胎儿不同部位皮肤组织中的表达,并加以图像分析. 结果: Ck10可见于所有标本的表皮基底层以上细胞中,在成人皮肤中表达层数比胎儿皮肤中多,且Ck10在成人皮肤及胎儿皮肤中表达差异不显著;Ck19在部分表皮基底层细胞及毛囊细胞中表达,β1整合素仅存在于基底层细胞及部分毛囊细胞,且胎儿皮肤中阳性细胞数目要比成人多,β1整合素表达阳性率在胎儿与成人皮肤中有显著差异(P<0.05);胎儿各组间比较,头皮组平均灰度值约为113.9,与其他几组相比有显著性差异(P<0.05). 结论: 表皮干细胞分布于人类皮肤的表皮基底层及部分毛囊细胞中,在胎儿皮肤中该细胞数量要比成人多,表明该类细胞随年龄逐渐减少的生物学改变,也反映了皮肤的生理性变化.
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