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rhBMP-2-induced signal transduction in NIH3T3 fibroblasts
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 17
- DOI:
- 10.3321/j.issn:1000-2790.2003.17.005
- Key Word:
- 成纤维细胞;骨形成蛋白;信号转导
Abstract： 目的: 观察NIH3T3细胞中BMP信号转导因子的表达. 方法: 用免疫组化和原位杂交方法检测NIH3T3细胞中BMP,BMPⅠ,Ⅱ型受体和Smad1,4,5的表达,在细胞培养液中加入50 μg*L-1 rhBMP-2,检测细胞的碱性磷酸酶(ALP)活性和骨钙素(OC)含量观察细胞是否有成骨趋向. 结果: 细胞中有BMPⅠ,Ⅱ型受体和Smad1,4,5的表达;没有BMP的表达;加入50 μg*L-1 rhBMP-2后,ALP活性和OC含量均增高. 结论: rhBMP-2作用于NIH3T3细胞后,细胞有成骨趋向. NIH3T3细胞是rhBMP-2的靶细胞,细胞中有BMPs信号转导因子的表达.
- [1]C H, Heldin,K, Miyazono,P, ten Dijke.TGF-beta signalling from cell membrane to nucleus through SMAD proteins.[J].1997,390(6659).