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Radiation protection of platelet factor 4

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Author:
No author available
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
16
DOI:
10.3321/j.issn:1000-2790.2003.16.002
Key Word:
血小板因子4;造血系统;辐射防护;platelet factor 4;hematopoietic system;radiation protection

Abstract: AIM: To study the radiation protection effect of PF4 on mice from total body irradiation. METHODS: The experiments were divided into two parts. In part one, the mice were randomly divided into four groups. In part two, there were three groups. The total mice number was 70. Exposure group and protection group with PF4 were received total body irradiation with 7.5 Gy 60Coγ rays. The survival rate and survival days during 30 d were observed. On d 8 after irradiation, the number of peripheral blood cells was counted by automatic hemotopoietic cell counter. The number of bone marrow mononuclear cells and the forming ability of CFU-GM were measured. The content of DNA in bone marrow cells was measured with ultraviolet spectrophotometer. The change of cell cycle was assayed by flow-cytometry. The mass of body, spleen and thymus was measured respectively.RESULTS: PF4 could increase the survival rate of irradiated mice, increase the content of DNA and the number of bone marrow mononuclear cells, improve the forming ability of CFU- GM,reduce proliferation index of hemopoietic cells, and increase spleen/body ratio, and thymus/body ratio. CONCLUSION: The administration of PF4 before total body irradiation resulted in a significant abatement in the radiation damage of the bone marrow and lymphoid tissues. PF4 could be a radiation protective agent.

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