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Isolation and purification of total saponins from cornus officinalis by column chromatography of macroreticular resin
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 8
- DOI:
- 10.3321/j.issn:1000-2790.2003.08.005
- Key Word:
- 山茱萸;皂苷类;大孔吸附树脂
Abstract： 目的: 研究并优化大孔树脂法分离纯化山茱萸总皂苷.方法: 700 mL*L-1乙醇提取后,上HPD-300型大孔吸附树脂,水洗后分别用300 mL*L-1,500 mL*L-1,700 mL*L-1乙醇洗脱,考察大孔树脂富集、纯化山茱萸总皂苷的吸附性能和洗脱参数,并与常规溶剂提取法相对照.结果: 山茱萸总皂苷富集于500 mL*L-1乙醇洗脱液部分,洗脱剂用量为上柱样品液的8倍,洗脱率达81.8%.大孔树脂法与常规溶剂法提取山茱萸总皂苷的得率分别为2.49%,2.19%.结论: 采用大孔吸附树脂可较好地纯化山茱萸总皂苷.
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