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Effects of tetrandrine on apoptosis of cultured neonatal rat cardiomyocytes during ischemia/reperfusion injury
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.2003.04.005
- Key Word:
- 心肌缺血;再灌注;脱噬作用;粉防己碱
Abstract： 目的: 研究粉防己碱对培养心肌细胞模拟缺血/再灌过程中心肌细胞凋亡的影响. 方法: 采用SD大鼠乳鼠进行心肌细胞培养,建立模拟心肌缺血/再灌注模型,实验分3组:①正常对照组;②模拟缺血/再灌注组:细胞缺氧120 min,复氧240 min;③ 30 μmol·L-1粉防己碱+缺血/再灌注组. 计算台盼蓝摄取率,测定乳酸脱氢酶(LDH)活性,以流式细胞仪(FCM)和透射电镜观察细胞凋亡. 结果: 在正常对照组,FCM未发现凋亡峰,电镜未发现凋亡细胞,而缺血120 min/再灌注240 min组FCM的凋亡率为15.1%,电镜可见到典型的凋亡细胞,台盼蓝摄取率和LDH活性显著增加(P<0.01). 粉防己碱组则细胞大部分存活,凋亡率明显降低(平均3.2%). 结论: 细胞凋亡参与了心肌缺血/再灌注损伤,粉防己碱可减少缺血/再灌注损伤中的心肌细胞凋亡.
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