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Two-dimensional electrophoresis and mass spectrometry in proteomics research

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Author:: No author available

Journal Title:: JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY

Issue:: 22

DOI:: 10.3321/j.issn:1000-2790.2002.22.001

Key Word:: 双向电泳;蛋白质类/分析;光谱分析,质量

Abstract：蛋白组分析是对生物蛋白组的分析与检测,即对生物蛋白质的大范围研究. 蛋白组分析首先要将混合蛋白质分离,分离的主要方法是蛋白双向电泳. 蛋白双向电泳是一种高效且广泛应用于分析由细胞、组织或其他生物样本中提取的复杂蛋白混合物的方法. 此方法根据蛋白互不关联的两个特性-等电点和分子量对蛋白进行分离. 它分辨率高,稳定性好,是蛋白组分析的关键技术之一. 已被分离的蛋白质由质谱分析对其进行鉴定. 质谱测量主要有两种方式:基质辅助激光解吸电离/飞行时间质谱测量法和电子喷雾电离质谱测量法. 此两种方法操作方式不同,但其所获得的信息互为补充. 前者以多肽质量/电荷比为依据同数据库资料进行比较,进而对蛋白进行鉴定,此法通常被称为多肽质量指纹分析. 后者由离子谱推得多肽的氨基酸序列,并依据这些氨基酸序列进行蛋白鉴定,此法较多肽质量指纹分析鉴定更准确、可靠. 蛋白组分析可用于样本全蛋白检测,样本蛋白表达检测,蛋白相互作用检测和蛋白修饰检测等方面,具有广阔的前景.

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