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Experimental study on construction of tissue-engineered heart valve using porcine decellular aortic valve
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 20
- DOI:
- 10.3321/j.issn:1000-2790.2002.20.002
- Key Word:
- 猪;主动脉瓣;心脏瓣膜,人工
Abstract： 目的观察未经戊二醛处理的去细胞猪主动脉瓣组织相容性和可降解性,并探讨其构建组织工程瓣可行性. 方法应用体外种植犬主动脉壁间质细胞和内皮细胞的去细胞猪主动脉瓣叶种植于犬腹主动脉内,观察其形态学和免疫组织化学变化. 结果①皮下埋藏实验显示瓣叶轻度炎症反应,10 wk埋藏物完全降解吸收;②体外细胞种植后7 d,见瓣叶表面有单层种子细胞覆盖;③体内,即腹主动脉内移植瓣叶,支架逐渐吸收,为新生细胞综合和分泌的纤维结缔组织所取代,至10 wk末瓣叶完全重塑,在新构成的瓣叶中可检测到Ⅰ, Ⅲ型胶原、弹力纤维和粘多糖,间质细胞部分呈α-平滑肌肌动蛋白阳性表达,内皮细胞覆盖于瓣叶表面,Ⅷ因子染色为阳性. 结论去细胞猪主动脉瓣体外初步构建的组织工程瓣,移植入犬腹主动脉内经10 wk观察,已初步形成具有活细胞的瓣叶组织,并提示组织相容性优于人工合成材料.
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