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Effects of inhaled nitric oxide on the expression of nitric oxide synthase in rabbit reperfused lung
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- 10.3321/j.issn:1000-2790.2002.18.003
- Key Word:
- 一氧化氮;再灌注;肺;一氧化氮合酶
Abstract： 目的探讨再灌注时吸入一氧化氮(NO)对兔肺一氧化氮合酶(NOS)同功酶表达的影响. 方法 40只健康新西兰大白兔随机分成4组,每组10只. 假手术组(Ⅰ组);假手术+吸入NO组(Ⅱ组);缺血再灌注组(Ⅲ组);缺血再灌注+吸入NO组(Ⅳ组). 通过阻断左肺门60 min,随即阻断右肺门,造成左肺单侧再灌注240 min. Ⅱ, Ⅳ组在阻断右肺门前5 min吸入50×10-6 NOppm. 再灌注240 min后,各组取左下叶组织,采用免疫组化方法检测再灌注肺NOS同功酶表达变化. 结果①Ⅰ, Ⅱ组肺组织未见iNOS的表达,eNOS在内皮细胞表达正常. ②Ⅲ组内皮细胞、肺泡上皮细胞、平滑肌细胞等均可见大量iNOS表达(分别为91.67%,83.3%和81.6%. 与Ⅰ相比P<0.05),eNOS表达下调(56.57%. 与Ⅰ相比P<0.05). Ⅳ组肺组织iNOS表达受抑制,eNOS表达正常. 结论①再灌注肺组织eNOS表达减弱,iNOS表达增强,导致NO合成增多,造成肺组织缺血灌注损伤. ②吸入NO能抑制肺组织iNOS表达,维持eNOS在内皮细胞的正常表达,减轻肺再灌注损伤.
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