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Quantitative analysis of HCV-RNA of different genotypes
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 17
- DOI:
- 10.3321/j.issn:1000-2790.2002.17.004
- Key Word:
- 肝炎病毒,丙型;RNA定量;HCV基因型;荧光定量PCR
Abstract： 目的根据定量PCR检测结果,了解血清不同基因型HCV-RNA的含量. 方法采用荧光定量PCR和逆转录巢式定性PCR同时检测166例丙肝患者的血清HCV-RNA,再进行酶切分型. 计算各型HCV-RNA平均含量. 结果定量PCR阳性血清(131例)HCV-RNA含量在1×104.04～1×108.11拷贝*mL-1. HCV-Ⅱ型(106例)、HCV-Ⅲ型(18例)和Ⅱ/Ⅲ混合型(3例)RNA平均滴度分别为1×106.88±2.01拷贝*mL-1,1×105.79±1.82拷贝*mL-1和105.50±1.62拷贝*mL-1. 结论定性和定量检测结果有很好的一致性. HCV-Ⅱ型病毒含量显著高于HCV-Ⅲ型.
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