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Study of therapeutic time window for Shenfu solution in transient focal cerebral ischemic injury in rats
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 15
- DOI:
- 10.3321/j.issn:1000-2790.2002.15.004
- Key Word:
- 参附注射液;脑缺血;治疗时间窗
Abstract： 目的探讨参附注射液对局灶性脑缺血性损伤的治疗时间窗. 方法 80只雄性SD大鼠,随机分为8组(每组 n =10):对照组,即单纯缺血再灌注组;参附注射液治疗A～G组,分别于缺血再灌注后0,0.5,1.0,1.5,2.0,4.0和6.0 h经腹腔注射参附注射液,剂量为10 mL*kg-1. 用右侧颈内动脉线栓法致大脑中动脉阻闭120 min,拔出尼龙线即刻为再灌注时间. 观察再灌注后24 h时神经功能损害改变并评分,24 h时处死动物,取大脑行TTC染色以测量脑梗死容积. 结果 24 h时神经功能损害评分,A～F组分别为: 0.5±0.3,0.5±0.4,0.6±0.5,0.8±0.4,0.8±0.3和0.9±0.3,明显低于对照组(2.1±0.8)及G组(1.5±0.7) ( P <0.05). 脑梗死容积A～F组分别为(46.4±26.1),(60.2±25.8),(67.2±36.7),(76.0±39.7),(94.1±34.9)和(98.4±35.3) mm3;均明显小于对照组(182.6±39.6) mm3及G组(139.1±42.8) mm3 ( P <0.05),A～F组间24 h时神经功能损害评分和梗死容积无差别( P >0.05). 结论参附注射液对局灶性脑缺血再灌注损伤的最佳治疗时机在缺血后4 h以内.
- [1].参附注射液对兔脊髓缺血损伤的保护作用[J].2000,(3).
- [2]Hakim A. M..Ischemic penumbra: The therapeutic window[J].1998.
- [3]Z G, Zhang,M, Chopp,W X, Tang,etc.Postischemic treatment (2-4 h) with anti-CD11b and anti-CD18 monoclonal antibodies are neuroprotective after transient (2 h) focal cerebral ischemia in the rat.[J].1995,698.
- [4]J M, Wardlaw,C P, Warlow,C, Counsell.Systematic review of evidence on thrombolytic therapy for acute ischaemic stroke.[J].1997,350.
- [5]G, Zhong,Y, Jiang.Calcium channel blockage and anti-free-radical actions of ginsenosides.[J].1997,110.
- [6]Dyker AG,Lees KR.Duration of neuroprotective treatment for ischemic stroke.[J].1998,29(2).
- [7]J A, Zivin.Factors determining the therapeutic window for stroke.[J].1998,50.
- [8]Ueta N,Kitahara S,Kubota M,etc.Therapeutic time window in the penumbra during permanent focal ischemia in rats: changes of free fatty acids and glycerophospholipids.[J].2000,22(4).
- [9]Longa E. Z.,Weinstein P. R.,Carlson S.,etc.Reversible middle cerebral artery occlusion without craniectomy in rats[J].1989.
- [10]J.-H Lim,T.-C Wen,S Matsuda,etc.Protection of ischemic hippocampal neurons by ginsenoside Rb1, a main ingredient of ginseng root[J].1997,28(3).
- [11]Linnik MD,McCarty DR,Johnson MP,etc.Six-hour window of opportunity for calpain inhibition in focal cerebral ischemia in rats.[J].1998,29(1).
- [12]Markarian GZ,Lee JH,Stein DJ,etc.Mild hypothermia: therapeutic window after experimental cerebral ischemia.[J].1996,38(3).
- [13]Gregory J. del Zoppo,Simone Wagner,Masafumi Tagaya.Trends and Future Developments in the Pharmacological Treatment of Acute Ischaemic Stroke[J].1997,54(1).
- [14].Tissue Plasminogen Activator for Acute Ischemic Stroke.[J].1995,333(24).
- [15]张英鸽,刘天培.人参皂苷Rb1和Rg1对大鼠可逆性局灶性脑缺血的影响[J].1996,(1).