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Neuroprotection of preconditioning with oxygen inhalation against ischemic injury in transient middle cerebral artery occlusion rats

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Author:: No author available

Journal Title:: JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY

Issue:: 15

DOI:: 10.3321/j.issn:1000-2790.2002.15.003

Key Word:: 脑缺血;脑梗塞;缺血预处理;氧吸入疗法;大鼠

Abstract：目的研究吸入不同时间100% O2能否诱导脑缺血耐受而对脑缺血再灌注损伤产生保护作用. 方法 63只SD大鼠随机分为7组(每组 n =9):即A组(对照组)于缺血前24 h吸空气24 h;B,C和D组分别于缺血前24 h连续吸100% O2 24,12和6 h;E,F和G组分别连续吸100% O2 24,12和6 h. A, B, C和D组于吸氧结束后24 h造成脑缺血;E组于吸氧后即刻造成脑缺血模型;F组间隔12 h; G组间隔18 h后造成脑缺血. 局灶性脑缺血模型采用3-0尼龙线线栓法栓塞大脑中动脉,2 h后拔出尼龙线使血液再灌注. 脑缺血2 h和再灌注后24 h行脑功能障碍评分,并处死动物取大脑行TTC染色测量脑梗死容积. 结果 B组的脑功能障碍评分与A组相比显著降低( P <0.05),C,D,E,F和G组与A组相比无显著性差异;脑梗死容积B组(104.8±62.1) mm3显著小于A组(199.0±69.6) mm3 ( P <0.01). 吸100% O2 12 h及6 h组及吸100% O2 24 h后即刻造成脑缺血组与对照组相比其脑梗死容积无明显差异. 结论本实验首次证实连续吸100% O2 24 h且间隔一定时间后能模拟缺血预处理对大鼠短暂性脑缺血产生保护作用.

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