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Tissue engineering bone with higher porousity coralline as scaffolds
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1000-2790.2002.10.004
- Key Word:
- 珊瑚;骨髓细胞;支架;生物相容性材料
Abstract： 目的探讨西沙群岛高孔隙率滨珊瑚作为骨组织工程支架材料的可行性,为组织工程研究开辟新的途径. 方法取4 wk龄兔骨髓,分离骨髓基质细胞,体外培养,经诱导分化为成骨细胞,接种至滨珊瑚材料,无菌条件下植入裸鼠皮下组织中. 对照组单纯植入滨珊瑚. 分别于4, 8 wk取材,行大体观察、X线摄影及组织学染色,观察新骨形成情况. 结果 4 wk时X线片有高密度阻射影像,HE染色可见有新骨形成;8 wk时X线阻射影像密度更高,HE染色可见大量新骨形成并相互连接成骨小梁样结构,骨细胞位于陷窝中. 结论高孔隙率滨珊瑚可以作为骨组织工程的支架材料并有广阔的应用前景.
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