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Effect of PDGFR-β antisense oligonucleotides on proliferation of cultured rat aortic smooth muscle cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 7
- DOI:
- 10.3321/j.issn:1000-2790.2002.07.004
- Key Word:
- 反义寡核苷酸;血管平滑肌细胞;再狭窄;血小板衍化生长因子;受体;细胞核增殖抗原
Abstract： 目的研究血小板衍化生长因子β受体(PDGFR-β)反义寡核苷酸(AODN)对血管平滑肌细胞(VSMC)增殖的影响.方法建立大鼠血管平滑肌细胞体外增殖模型.将细胞分为反义寡核苷酸组、正义寡核苷酸组、无义寡核苷酸组和空白对照组,其中反义组按浓度又分为1,2.5,5,10和15 μmol·L-1 5小组.在加药后24,48和72 h以流式细胞仪测定细胞周期,免疫细胞化学染色分析细胞核增殖抗原的表达,MTT(四唑盐)比色法测定细胞增殖活力.结果 10 μmol·L-1 PDGFR-β AODN干预VSMC 48 h后,处于DNA合成前期(G0/G1)细胞数分数(0.70)高于空白对照组(0.07,P<0.05);PCNA免疫细胞化学染色发现以5～15 μmol·L-1 PDGFR-β AODN干预48 h后VSMC各组胞核染色强度为20.4%～6.4%明显弱于空白组(73.8%),正义组(72.9%)和无义组(75.6%)(P<0.05);10 μmol·L-1 PDGFR-β AODN作用48 h后VSMC的增殖抑制率为66.7%,高于正义组(8.1%),错义组(11.8%)和5 μmol·L-1 AODN组(45.4%) (P<0.05);同时10 μmol·L-1 AODN组48 h增殖抑制率与24 h(9.1%)相比明显增加(P<0.05).结论 PDGFR-β AODN对VSMC增殖有明显抑制作用,并且呈时间和浓度依赖性.
- [1]谈世进,金立仁,陈万春.血管舒缓素对PDGF诱导的大鼠血管平滑肌细胞增殖的影响[J].1999,(2).
- [2]王宏光,李开宗,窦科峰.反义寡核苷酸抑制肝癌细胞血管内皮生长因子的表达[J].2000,(11).
- [3]Waltenberger J.,Uecker A.,Kroll J.,etc.A Dual Inhibitor of Platelet-Derived Growth Factor\u00a0-Receptor and Src Kinase Activity Potently Interferes With Motogenic and Mitogenic Responses to PDGF in Vascular Smooth Muscle Cells: A Novel Candidate for Prevention of Vascular Remodeling[J].1999.
- [4]C, Espinola-Klein,H J, Rupprecht,R, Erbel,etc.Impact of restenosis 10 years after coronary angioplasty.[J].1998,19(7).
- [5]Avino AJ,Bandyk DF,Gonsalves C,etc.Duplex features of vein graft stenosis and the success of percutaneous transluminal angioplasty.[J].1999,6(1).
- [6]C E, Hart,A W, Clowes.Platelet-derived growth factor and arterial response to injury.[J].1997,95.
- [7]高炜.再狭窄的细胞和分子生物学（一）[J].1997,(2).