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Induction and histopathological study of lymphangiomas in mice
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.2002.04.003
- Key Word:
- 淋巴管瘤/病理学;疾病模型,动物;小鼠
Abstract： 目的建立重复性好的小鼠淋巴内皮细胞构成的良性肿瘤模型. 方法 BALB/c小鼠20只腹腔注射弗氏不完全佐剂2次, 间隔15 d, 处死后对诱导出的腹腔新生组织进行组织学观察, 并用免疫组织化学方法检测淋巴内皮细胞标志性抗原VEGFR-3和CD31. 结果实验小鼠中有18只(90%)膈肌腹腔面及肝脏表面出现边界清楚的多中心性的白色肿瘤样组织, 光镜及透射电镜下为多管腔囊性, 由内皮细胞构成,并表达CD31及VEGFR-3. 观察6 mo肿瘤有自限性. 结论弗氏不完全佐剂腹腔注射可稳定诱导小鼠腹腔淋巴管瘤.
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