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Combination of Coxsackievirus B3 monoclonal antibody with cardiac myocytes and its pathopoiesis
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1000-2790.2002.01.005
- Key Word:
- 柯萨奇B3病毒;心肌细胞;单克隆抗体;免疫组化
Abstract： 目的研究柯萨奇B3病毒单克隆抗体(CVB3-mAb) 与心肌细胞结合 ,以探讨CVB3-mAb对心肌细胞的致病作用. 方法采用细胞融合技术制备CVB3-mAb,并应用各细胞株分泌的mAb与人、牛、大鼠心肌细胞进行免疫组化分析,比较各株分泌的CVB3-mAb与不同种动物心肌细胞间结合的差异. 结果利用细胞融合技术制备出26种CVB3-mAb. 其部分CVB3-mA b可与心肌细胞膜或心肌细胞内的肌原纤维结合,呈棕黄色环状重叠或横纹. CVB3-mAb 2A4,2D4,1G6,1H9,1H10,1H11与人、牛、大鼠心肌细胞均呈阳性反应;CVB3-mAb 2A2仅与人的心肌细胞结合;CVB3-mAb 2B2,2H4,1C10,1G4与人及大鼠心肌细胞结合;CVB3-mAb 2A3与牛和大鼠心肌细胞结合;CVB3-mAb 2G12仅与大鼠心肌细胞结合;CVB3-mAb 1E8仅与牛的心肌细胞结合. 且CVB3-mAb 2D4与人心肌的结合为心肌细胞膜,而与牛及大鼠心肌的结合为心肌细胞内的肌原纤维. 结论各CVB3-mAb与不同动物心肌细胞间的结合及结合形式不同;且同一动物的心肌细胞与CVB3-mAb结合有差异. 部分柯萨奇B3病毒抗体与动物心肌细胞结合,可引起心肌炎.
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