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Cloning of anti-keratin IgM antibodies from a human natural antibody library
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 24
- DOI:
- 10.3321/j.issn:1000-2790.2001.24.005
- Key Word:
- ZGM;噬菌体;抗体,细菌;角蛋白
Abstract： 目的克隆制备人源性抗角蛋白IgM抗体. 方法以人表皮角蛋白为抗原,从人源性天然抗体库中筛选IgM型抗角蛋白人抗体,并用ELISA对所获抗体进行抗原结合活性和特异性等鉴定. 结果通过3轮"亲和吸附-洗脱-扩增"筛选,获得2株IgM型抗角蛋白噬菌体抗体. 酶切去掉载体上的噬菌体基因Ⅲ,得到了可溶性表达的Fab段. 经过鉴定,所获抗体具有良好的抗原结合活性和特异性. 结论从人源性天然抗体库中成功筛选出2株抗角蛋白IgM抗体,为进一步研究抗角蛋白抗体的特性提供了条件.
- [1]夏汝山,刘玉峰,李承新,等.同种异体抗角蛋白自身抗体对家兔接触性皮炎模型IL-6,IL-8的影响[J].2001,(15).
- [2]王刚,化冰,王琰,等.从半合成噬菌体抗体库中筛选抗角蛋白人抗体[J].2000,(1).
- [3]A, Coutinho,M D, Kazatchkine,S, Avrameas.Natural autoantibodies.[J].1995,7(6).
- [4]Hintner, Helmut,Romani, Nikolaus,Stanzl, Ursula,etc.Phagocytosis of Keratin Filament Aggregates Following Opsonization With IgG-Anti-Keratin Filament Autoantibodies.[J].1987,88(2).
- [5]Reurs A,Roovers RC,Hufton SE,etc.A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies.[J].1999,274(26).
- [6]S. Avrameas.Natural autoantibodies: from ‘horror autotoxicus’ to ‘gnothi seauton’[J].1991,12(5).
- [7]Lacroix-Desmazes S,Malanchere E,Kazatchkine MD,etc.Self-reactive antibodies (natural autoantibodies) in healthy individuals.[J].1998,216(1).
- [8]Stratis Avrameas,Thérèse Ternynck.The natural autoantibodies system: Between hypotheses and facts[J].1993,30(12).
- [9]Y F, Liu,Y F, Liu,T S, Zhang,etc.Immunohistochemical study of anti-keratin autoantibodies in both normal and pathological state.[J].1993,106(5).