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Effect of anti-keratin autoantibody on proliferation of melanocytes and keratinocytes cultured with serum-free medium
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 24
- DOI:
- 10.3321/j.issn:1000-2790.2001.24.004
- Key Word:
- 角蛋白;自身抗体;黑素细胞;角蛋白细胞
Abstract： 目的建立一种无血清传代培养黑素细胞的方法,并观察抗角蛋白自身抗体(AK auto Ab)对无血清培养黑素细胞及角质形成细胞增殖的影响. 方法应用角质形成细胞无血清培养基(KC-SFM)添加牛垂体浸出液(BPE)和TPA传代培养黑素细胞,应用KC-SFM添加EGF和BPE传代培养角质形成细胞,培养均至第3代时于培养基中加入不同浓度的AK auto Ab,用MTT法观察AK auto Ab对两种细胞增殖的影响. 结果应用无血清培养基成功地传代培养了黑素细胞,MTT法结果显示AK auto Ab各浓度组对培养黑素细胞的增殖无明显影响,但对角质形成细胞的增殖有显著抑制作用并有浓度依赖性. 结论建立了一种无血清培养黑素细胞的方法,AK auto Ab对黑素细胞的增殖无抑制作用而对角质形成细胞有特异性抑制作用.
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