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Analysis of the self-reactivity of IgM anti-keratin autoantibodies in healthy human sera at different ages
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 24
- DOI:
- 10.3321/j.issn:1000-2790.2001.24.003
- Key Word:
- 角蛋白;自身抗体;反应模式
Abstract： 目的分析正常人血清IgM类抗角蛋白自身抗体(AK auto Abs)的自身反应特性及其与年龄的相关性. 方法应用间接ELISA法检测新生儿(脐带血)、幼儿、成年人及老年人血清IgM类AK auto Abs的滴度,免疫印迹法检测抗体对自身角蛋白的反应特征,并对结果进行比较分析. 结果脐带血中存在着IgM类AK auto Abs,但其滴度明显低于其他三个年龄组,而其他三组的抗体滴度无明显差异(P>0.05);新生儿组IgM类AK auto Abs对角蛋白的反应模式在不同个体之间均匀一致,在其他三组不同个体之间有一定异质性,但不同年龄组之间无相关性. 结论 IgM类AK auto Abs天然存在于正常人体内,但出生时滴度较低,反应模式较为一致;幼年期抗体水平迅速增加,反应模式趋向多样化;以后各年龄段基本保持稳定.
- [1]V, Hurez,S V, Kaveri,M D, Kazatchkine.Expression and control of the natural autoreactive IgG repertoire in normal human serum.[J].1993,23(4).
- [2]L, Mouthon,S, Lacroix-Desmazes,A, Nobrega,etc.The self-reactive antibody repertoire of normal human serum IgM is acquired in early childhood and remains conserved throughout life.[J].1996,44(3).
- [3]R, Eichner,T T, Sun,U, Aebi.The role of keratin subfamilies and keratin pairs in the formation of human epidermal intermediate filaments.[J].1986,102(5).
- [4]Kazatchkine MD,Mouthon L,Lacroix Desmazes-S,etc.Analysis of human self-reactive antibody repertoires by quantitative immunoblotting.[J].2000,240(1).
- [5]Y, Tomer,Y, Shoenfeld.Ageing and autoantibodies.[J].1988,1(2).