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Inhibition effects of human gliomas by direct tumor injection of eukaryotic vector encoding endostatin gene
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 23
- DOI:
- 10.3321/j.issn:1000-2790.2001.23.003
- Key Word:
- 内皮抑素;胶质瘤;表达;治疗
Abstract： 目的探讨endostatin裸DNA对裸鼠皮下人脑胶质瘤生长的影响特点. 方法用脂质体包埋法将endostatin基因的真核表达载体pcDNA-S-Endo注入裸鼠皮下SHG44人脑胶质瘤内,观察肿瘤生长情况并计算抑瘤率,结合免疫组化、电镜、微血管和瘤体坏死区计数,确定endostatin裸DNA治疗人脑胶质瘤的特点. 结果 Endostatin裸DNA在荷瘤裸鼠瘤体内获得表达,裸鼠皮下胶质瘤血管生成能力显著下降,肿瘤生长受到抑制,抑瘤率达到41.6%. 结论 Endostatin裸DNA能够抑制人脑胶质瘤生长,它是通过抑制肿瘤血管生成,导致肿瘤坏死实现的. 本研究为应用裸DNA药物治疗人脑胶质瘤奠定了初步基础.
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