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Study on NIDRAN to induce the apoptosis of human brain glioma cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 23
- DOI:
- 10.3321/j.issn:1000-2790.2001.23.001
- Key Word:
- 宁得朗;凋亡;胶质瘤
Abstract： 目的探讨宁得朗(NIDRAN)对人脑胶质瘤细胞凋亡的诱导作用. 方法用含10 μmol*L-1 NIDRAN的RPMI 1640培养液,作用于SHG44细胞3 h,收集细胞用于形态学检测,DNA电泳及流式细胞分析. 结果①形态学观察:大部分瘤细胞体积缩小,核固缩,染色质浓缩在核模边缘呈月牙状聚集,有凋亡小体形成. ②DNA电泳:在800 bp以下可见明显的DNA梯状带. ③流式细胞分析:出现典型的细胞凋亡峰,凋亡细胞占细胞总数的32.1%. 结论 NIDRAN可诱导人胶质瘤细胞发生凋亡.
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