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Protective effects of electroacupuncture on acute gastric mucosal lesion and SS, NOS, VIP, CGRP under psychological stress in rats
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 22
- DOI:
- 10.3321/j.issn:1000-2790.2001.22.005
- Key Word:
- 电针;应激,心理学;胃粘膜/损伤;胃肠激素类
Abstract： 目的探讨电针对心理性应激状态下胃粘膜损伤的保护作用的机制. 方法将96只雄性SD大鼠随机分成3组,A:正常对照组;B:心理性应激组;C:心理性应激+针刺组. 采用高压恒流脉冲刺激器制备心理性应激模型. 电针刺激大鼠双侧足三里,用Chen法测定胃粘膜损伤指数并计算保护率. 用放免分析法、生化法同步测定各组血浆中SS,NOS,CGRP,VIP的含量. 结果心理应激条件下,B组胃粘膜出现损害、甚至产生溃疡,且随着应激反应程度的不断增强,胃粘膜损害的程度不断加大,而C组胃粘膜损伤明显减轻,且随着时间的延长,这种效果更加明显.胃粘膜保护率也增加. 大鼠血浆SS的浓度B组显著高于A组及C组(P<0.01),B组与C组SS有明显差异(P<0.01). NOS的活性,B组明显高于A组(P<0.01).C组与B组也有明显差异(P<0.05). 但是,A组与C组二者无差异(P>0.05). CGRP的水平在B组发生很大的变化. 随着应激程度的不断加强,CGRP的水平不断升高,与A组相比有明显差异(P<0.01). 然而,C组与A组相比无明显差异(P>0.05),但与B组相比差异显著(P<0.01). 心理性应激状况下,VIP水平的明显升高. B组与A组相比差异明显(P<0.01),C组VIP的水平与A组无明显差异(P>0.05),而与B组差异明显(P<0.05). 结论心理性应激导致胃粘膜的损伤及电针穴位对其产生调节防治作用的机制与某些胃肠道激素或神经肽密切相关.
- [1]B M, Evers,D, Parekh,C M, Townsend,etc.Somatostatin and analogues in the treatment of cancer. A review.[J].1991,213(3).
- [2]Zen Itoh,Akiyoshi Mizumoto,Yuji Iwanaga,etc.Involvement of 5-hydroxytryptamine 3 receptors in regulation of interdigestive gastric contractions by motilin in the dog[J].1991,100(4).
- [3]Knowles R G,Moncada S.Nitric oxide synthases in mammals[J].1994.
- [4]Amor Hajri,Marc Aprahamian,Christiane Damgé.Effect of prolonged administration of long-acting somatostatin on caerulein, CCK-8 and GRP induced pancreatic growth in the rat[J].1991,32(2).
- [5]J. B. Furness,Z. S. Li,H. M. Young,etc.Nitric oxide synthase in the enteric nervous system of the guinea-pig: a quantitative description[J].1994,277(1).
- [6]Pytko Polonczyk J,Konturek SJ,Brzozowski T,etc.Gastric adaptation to stress: role of sensory nerves, salivary glands, and adrenal glands.[J].1995,30(1).
- [7]K, Takeuchi,K, Takehara,T, Kaneko,etc.Nitric oxide and prostaglandins in regulation of acid secretory response in rat stomach following injury.[J].1995,272.
- [8]雷银雪,李兆申,湛先保,等.降钙素基因相关肽在大鼠应激性溃疡发生中的作用[J].1998,(5).