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Effects of acidosis on the L-type calcium current of rat ventricular myocytes
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILTARY MEDICAL UNIVERSITY
- Issue:
- 21
- DOI:
- 10.3321/j.issn:1000-2790.2001.21.004
- Key Word:
- 酸中毒;钙通道;大鼠;心肌细胞
Abstract： 目的观察酸中毒对心肌细胞L型钙通道电流(ICa*L)的影响. 方法采用全细胞膜片箝技术记录ICa*L. 用酸液灌流造成细胞外和细胞内酸中毒,NH4Cl洗脱造成单纯细胞内酸中毒. 结果细胞外酸中毒可明显抑制ICa*L, 在1～2 min内,pH 6.5及pH 7.0的灌流液对ICa*L的峰值抑制分别达正常的(41.7±7.5)%和(65.6±6.3)% (n=5,P<0.01), NH4Cl洗脱引起单纯细胞内酸中毒后,ICa*L被抑制到正常幅度的(69.4±5.8)% (n=5, P<0.01). 结论酸中毒对心肌细胞L型钙通道具有明显的抑制作用,细胞内酸中毒参与该作用.
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