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Biological characteristics of human cystic cancer cell line T 24 with higher HSP70 expression
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 20
- DOI:
- 10.3321/j.issn:1000-2790.2001.20.005
- Key Word:
- 膀胱肿瘤;热休克蛋白质类70;克隆,分子;T24细胞;细胞培养
Abstract： 目的筛选耐热T24细胞亚克隆,研究其生物学特性变化. 方法用热暴露筛选,联合应用免疫荧光化学方法及流式细胞仪筛选高表达HSP70的T24细胞亚克隆,计数法测定其细胞生长曲线及细胞倍增时间,流式细胞仪分析其细胞周期的变化. 分子生物学方法及细胞化学方法观察有无凋亡出现. 结果有限稀释法筛选获取耐热T24单克隆细胞株(HrT24)共26株 ,应用免疫荧光法筛选出HSP70阳性克隆12株,取荧光较强的两株命名为HrT24e,HrT 24j,FCM(平均荧光强度为0.29)检测HSPs分子的阳性表达率T24,HrT24e ,HrT 24j分别为2.4%,37.6%,65.8%. 细胞周期分析显示T24,HrT24e,HrT 24j增殖指数(proliferation index, PI 分别为44.3%, 39.0%, 38.5%, HrT24e与T24相比差异有显著意义(Yates correct ed, χ2=5.56, P=0.0183),HrT24e与T24j相比差异无显著意义(Yates corrected, χ2=0.03, P=0.8543). HrT24e与HrT24j凋亡率分别为4.7%, 5.9%. 分子生物学方法观察到DNA凋亡梯带出现,细胞化学方法观察到凋亡小体. [ HTH〗结论 HrT24细胞HSP70表达增加,出现了一定的增殖抑制现象,同时观察到细胞凋亡,推测它们有较好的免疫原性.
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